Erythrocyte shape analysis by means of laser diffraction

Rainer Bayer; Burkhard Schauf; Bernd Guenther

doi:10.1117/12.59367

6 May 1992 Erythrocyte shape analysis by means of laser diffraction

Rainer Bayer, Burkhard Schauf, Bernd Guenther

Proceedings Volume 1641, Physiological Monitoring and Early Detection Diagnostic Methods; (1992) https://doi.org/10.1117/12.59367
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

In quite a large number of disorders, reduced flexibility of red blood cells (RBC) can be detected. In cardiovascular diseases it is supposed that rigidification of RBC may be regarded as a pathogenetic factor aggravating ischemia by disturbing capillary perfusion. Most methods established so far to estimate RBC deformability are hard to standardize and include large measurement errors. We present a low-cost system to determine RBC shape and flexibility. It combines laser diffraction of RBC in Couette flow with automated computer assisted image analysis. Effortless handling allows the system to be used for RBC elongation measurements even in routine diagnostics. Analysis of the whole information content of diffraction patterns reduces errors due to noisy diffraction patterns of working a little off axis. The system allows detection of very small changes in flexibility (less than 5%). The accuracy of measurement is not affected by variation of hematocrit or the intensity of transmitted light. Using the newly developed system it is demonstrated (1) that mechanically induced RBC rigidification may occur without hemolysis; (2) that in photodynamic therapy (e.g., pheophorbide A) RBC rigidification occurs during irridation; and (3) that in-vitro aging of conserved blood may partly be inhibited by calmodulin antagonists (e.g., fendiline).

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Rainer Bayer, Burkhard Schauf, and Bernd Guenther "Erythrocyte shape analysis by means of laser diffraction", Proc. SPIE 1641, Physiological Monitoring and Early Detection Diagnostic Methods, (6 May 1992); https://doi.org/10.1117/12.59367

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