Laser-scanned fluorescence of nonlased/normal, lased/normal, nonlased/carious, and lased/carious enamel

Kenneth L. Zakariasen D.D.S.; Joseph R. Barron; Barry E. Paton

doi:10.1117/12.137381

1 June 1992 Laser-scanned fluorescence of nonlased/normal, lased/normal, nonlased/carious, and lased/carious enamel

Kenneth L. Zakariasen D.D.S., Joseph R. Barron, Barry E. Paton

Proceedings Volume 1643, Laser Surgery: Advanced Characterization, Therapeutics, and Systems III; (1992) https://doi.org/10.1117/12.137381
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Research has shown that low levels of CO₂ laser irradiation raise enamel resistance to sub-surface demineralization. Additionally, laser scanned fluorescence analysis of enamel, as well a laser and white light reflection studies, have potential for both clinical diagnosis and comparative research investigations of the caries process. This study was designed to compare laser fluorescence and laser/white light reflection of (1) non-lased/normal with lased/normal enamel and (2) non-lased/normal with non-lased/carious and lased/carious enamel. Specimens were buccal surfaces of extracted third molars, coated with acid resistant varnish except for either two or three 2.25 mm² windows (two window specimens: non-lased/normal, lased/normal--three window specimens: non-lased/normal, non-lased carious, lased/carious). Teeth exhibiting carious windows were immersed in a demineralizing solution for twelve days. Non-carious windows were covered with wax during immersion. Following immersion, the wax was removed, and fluorescence and laser/white light reflection analyses were performed on all windows utilizing a custom scanning laser fluorescence spectrometer which focuses light from a 25 mWatt He-Cd laser at 442 nm through an objective lens onto a cross-section >= 3 (mu) in diameter. For laser/white light reflection analyses, reflected light intensities were measured. A HeNe laser was used for laser light reflection studies. Following analyses, the teeth are sectioned bucco-lingually into 80 micrometers sections, examined under polarized light microscopy, and the lesions photographed. This permits comparison between fluorescence/reflected light values and the visualized decalcification areas for each section, and thus comparisons between various enamel treatments and normal enamel. The enamel specimens are currently being analyzed.

Citation Download Citation

Kenneth L. Zakariasen D.D.S., Joseph R. Barron, and Barry E. Paton "Laser-scanned fluorescence of nonlased/normal, lased/normal, nonlased/carious, and lased/carious enamel", Proc. SPIE 1643, Laser Surgery: Advanced Characterization, Therapeutics, and Systems III, (1 June 1992); https://doi.org/10.1117/12.137381

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