Morphological image processing and network analysis of cornea endothelial cell images

Luc M. Vincent; Barry R. Masters

doi:10.1117/12.60644

1 June 1992 Morphological image processing and network analysis of cornea endothelial cell images

Luc M. Vincent, Barry R. Masters

Proceedings Volume 1769, Image Algebra and Morphological Image Processing III; (1992) https://doi.org/10.1117/12.60644
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

In this paper, we propose a robust and accurate method for segmenting grayscale images of corneal endothelial tissue. Its first step consists of the extraction of markers of the corneal cells using a dome extractor based on morphological grayscale reconstruction. Then, marker- driven watershed segmentation yields binary images of the corneal cell network. From these images, we derive histograms of the cell sizes and number of neighbors, which provide quantitative information about the condition of the cornea. We also construct the neighborhood graph of the corneal cells, whose granulometric analysis yields information on the distribution of cells with large number of neighbors in the tissue. Lastly, these results help us propose a model for the corneal cell death phenomenon. The numerical simulation of this model exhibits a very good match with our experimental results. This model not only allows us to refine our understanding of the phenomenon: combined with our results, it enables the estimation of the percentage of cells having died in a given corneal endothelial tissue.

Citation Download Citation

Luc M. Vincent and Barry R. Masters "Morphological image processing and network analysis of cornea endothelial cell images", Proc. SPIE 1769, Image Algebra and Morphological Image Processing III, (1 June 1992); https://doi.org/10.1117/12.60644

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available