Multiple multipole scattering of light in mitochondrial suspensions

Manfred D. Kessler; Martin Radina; Klaus H. Frank

doi:10.1117/12.154645

14 September 1993 Multiple multipole scattering of light in mitochondrial suspensions

Manfred D. Kessler, Martin Radina, Klaus H. Frank

Proceedings Volume 1888, Photon Migration and Imaging in Random Media and Tissues; (1993) https://doi.org/10.1117/12.154645
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

In a recent optical study two dimensional intensity patterns of light irradiated into suspensions of mitochondria were investigated. We designed our experimental model in such a way that the concentration of scattering particles was similar to concentrations found in tissues of humans and mammals. Earlier measurements performed in isolated and hemoglobin-free perfused rat liver and heart revealed that tissue spectra were strongly altered by light scattering when functional states of the two organs e.g. tissue oxygenation were changed. Based upon Mie's theory as well as our observations in perfused organs we concluded that an important factor able to induce sizeable changes in light scattering under physiological or pathophysiological conditions could be alterations in size of subcellular particles. The classical Mie theory which yields for single multipole scattering shows an increase in forward and backward scattering as a function of particle radius. E.g. when particle size is increased from a radius of 0.1 to 2 micrometers forward scattering is augmented by a factor of 273,000 while backward scattering attains a value of 13,900. Measurements of angular light distribution obtained in mitochondrial suspensions revealed first evidence that multiple multipole scattering increases backward scattering while forward scattering becomes diminished when particle size is enlarged.

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Manfred D. Kessler, Martin Radina, and Klaus H. Frank "Multiple multipole scattering of light in mitochondrial suspensions", Proc. SPIE 1888, Photon Migration and Imaging in Random Media and Tissues, (14 September 1993); https://doi.org/10.1117/12.154645

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KEYWORDS

Light scattering

Particles

Scattering

Mie scattering

Tissues

Multiple scattering

Liver

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