Computed alignment of serial sections for 3D reconstructions

Lyndon S. Hibbard; Robert A. Grothe Jr.; Tamara L. Arnicar-Sulze

doi:10.1117/12.148708

29 July 1993 Computed alignment of serial sections for 3D reconstructions

Lyndon S. Hibbard, Robert A. Grothe Jr., Tamara L. Arnicar-Sulze

Proceedings Volume 1905, Biomedical Image Processing and Biomedical Visualization; (1993) https://doi.org/10.1117/12.148708
Event: IS&T/SPIE's Symposium on Electronic Imaging: Science and Technology, 1993, San Jose, CA, United States

Abstract

Image alignment is an absolute requirement for creating three-dimensional reconstructions from serial sections. The rotational and translational components of misalignment can be corrected by an iterative correlation procedure, but for images having significant differences, alignment can fail with a likelihood proportional to the extent of the differences. We found that translational correction was much more reliably determined when lowpass filters were applied to the product transforms from which the correlations were calculated. Also, rotational corrections based on polar analyses of the images' autocorrelations instead of the images directly contributed to more accurate alignments. These methods were combined to generate 3-D reconstructions of brain capillaries imaged by transmission electron microscopy.

Citation Download Citation

Lyndon S. Hibbard, Robert A. Grothe Jr., and Tamara L. Arnicar-Sulze "Computed alignment of serial sections for 3D reconstructions", Proc. SPIE 1905, Biomedical Image Processing and Biomedical Visualization, (29 July 1993); https://doi.org/10.1117/12.148708

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available