Action of UV-A and blue light on enzymes activity and accumulation of lipid peroxidation products in attached and detached frog retinas

Victoria Alkseyevna Lapina; Alexander E. Dontsov

doi:10.1117/12.180819

21 July 1994 Action of UV-A and blue light on enzymes activity and accumulation of lipid peroxidation products in attached and detached frog retinas

Victoria Alkseyevna Lapina, Alexander E. Dontsov

Author Affiliations +

Proceedings Volume 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards; (1994) https://doi.org/10.1117/12.180819
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The effect of the UV-A and blue light on the accumulation of lipid peroxidation products and activities of succinate dehydrogenase and superoxide dismutase in the retina was examined in eye cup model of dark and light adapted frogs R. temporaria. Retinas were exposed to UV-A radiation (8 mW/cm²) and blue light (10 to 150 mW/cm²) for periods from 5 min to 1 hr. We have measured TBA-active products both in the retina homogenates and in the reaction media. Enzyme activities was measured in the retina homogenates only. The measurements revealed a significant increase in the endogenous and exogenous forms of lipid peroxidation products in the retina of dark adapted frog (1.6+/- 0.4; 1.4+/- 0.3 nmole TBA-active products per mg protein, respectively) compared to light adapted (0.85+/- 0.16; 0.32+/- 0.06 nmole TBA-active products per mg protein, respectively). In the same conditions succinate dehydrogenase activity was decline more than 50% but superoxide dismutase activity didn't decrease. Disorganized inner and outer segments were observed after 40 min exposures. No light microscopic changes were detected after 5 min exposures. Light damage was significantly higher in the retina of dark adapted frog. The results indicate that the retina from eye cup of dark adapted frog is more susceptible to UV-A and blue light damages.

Citation Download Citation

Victoria Alkseyevna Lapina and Alexander E. Dontsov "Action of UV-A and blue light on enzymes activity and accumulation of lipid peroxidation products in attached and detached frog retinas", Proc. SPIE 2134, Laser-Tissue Interaction V; and Ultraviolet Radiation Hazards, (21 July 1994); https://doi.org/10.1117/12.180819

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