In-situ histochemical analysis of human coronary artery by Raman spectroscopy compared with biochemical assay

James F. Brennan III; Tjeerd J. Roemer; Anna M. Tercyak; Yang Wang; Maryann Fitzmaurice; Robert S. Lees; John R. Kramer Jr.; Ramachandra R. Dasari; Michael S. Feld

doi:10.1117/12.208528

8 May 1995 In-situ histochemical analysis of human coronary artery by Raman spectroscopy compared with biochemical assay

James F. Brennan III, Tjeerd J. Roemer, Anna M. Tercyak, Yang Wang, Maryann Fitzmaurice, Robert S. Lees, John R. Kramer Jr., Ramachandra R. Dasari, Michael S. Feld

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208528
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

We have developed a method to analyze quantitatively the biochemical composition of human coronary artery in situ using near infrared Raman spectroscopy. Human coronary arteries were obtained from explanted hearts after heart transplantation. Samples of normal intima/media, adventitia, non-calcified and calcified plaque were illuminated with 830 nm light from a CW Ti:Sapphire laser. The Raman scattered light was collected and coupled into a 1/4 meter spectrometer that dispersed the light onto a liquid nitrogen cooled, deep-depletion CCD detector. Raman spectra with sufficiently high S/N for extracting biochemical information could be collected in under one second. The spectra were analyzed using a recently developed model to quantitate the relative weight fractions of cholesterol, cholesterol esters, triacylglycerol, phospholipids, protein, and calcium salts. After spectral examination, the artery samples were biochemically assayed to determine the total lipid weight and the amount of the major lipid categories as a percentage of the total lipid content. The results of the lipid biochemical assay and the Raman spectral model compare favorably, indicating that relative lipid weights can be accurately determined in situ. Protein and calcium salts assays are underway. This in situ biochemical information may be useful in diagnosing atherosclerosis and studying disease progression.

Citation Download Citation

James F. Brennan III, Tjeerd J. Roemer, Anna M. Tercyak, Yang Wang, Maryann Fitzmaurice, Robert S. Lees, John R. Kramer Jr., Ramachandra R. Dasari, and Michael S. Feld "In-situ histochemical analysis of human coronary artery by Raman spectroscopy compared with biochemical assay", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208528

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available