Endoscopic tissue fluorescence life-time imaging by frequency doamin light-induced fluorescence

Jerome C. Mizeret; Georges A. Wagnieres; A. Studzinski; C. Shangguan; Hubert van den Bergh

doi:10.1117/12.228902

27 December 1995 Endoscopic tissue fluorescence life-time imaging by frequency doamin light-induced fluorescence

Jerome C. Mizeret, Georges A. Wagnieres, A. Studzinski, C. Shangguan, Hubert van den Bergh

Proceedings Volume 2627, Optical Biopsies; (1995) https://doi.org/10.1117/12.228902
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

An instrumentation is being developed to draw a fluorescence life-time map of tissue endoscopically. This fluorescence life-time of an endogenous or exogenous fluorochrome gives information about the physico-chemical environment which is thought to vary between normal and diseased tissue. The excitation light from a cw laser is modulated in amplitude at high frequencies by an electro-optic modulator and delivered to the endoscopic site through an optical fiber. The image of the tissue is spectrally split in two parts, the one being the backscattered excitation light, the other the fluorescence of the fluorochromes. Each image is focused on the photocathode of an image intensifier whose gain is modulated at the same frequency. By acquiring frames at different phases between the excitation and the emission, it is possible to calculate pixel by pixel the apparent fluorescence life-time of the corresponding tissue region.

Citation Download Citation

Jerome C. Mizeret, Georges A. Wagnieres, A. Studzinski, C. Shangguan, and Hubert van den Bergh "Endoscopic tissue fluorescence life-time imaging by frequency doamin light-induced fluorescence", Proc. SPIE 2627, Optical Biopsies, (27 December 1995); https://doi.org/10.1117/12.228902

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available