Application of the method of partial least squares to determine chromophore concentrations from fluorescence spectra of turbid samples

Anthony J. Durkin; Rebecca R. Richards-Kortum

doi:10.1117/12.239536

10 May 1996 Application of the method of partial least squares to determine chromophore concentrations from fluorescence spectra of turbid samples

Anthony J. Durkin, Rebecca R. Richards-Kortum

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Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239536
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

An non-invasive optical method for obtaining quantitative biochemical information is desirable. While quantitative methods for predicting fluorophore concentrations in dilute solutions are well known, human tissue is highly scattering and absorbing and cannot be accurately treated with these techniques. We have employed the method of partial least squares (PLS) to develop an empirical, linear model of sample fluorescence from a training set with optical properties and known concentrations representative of those to be predicted. This model can be applied to predict chromophore concentrations in the unknown samples.

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Anthony J. Durkin and Rebecca R. Richards-Kortum "Application of the method of partial least squares to determine chromophore concentrations from fluorescence spectra of turbid samples", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239536

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KEYWORDS

Luminescence

Tissues

Calibration

Chromophores

Scattering

Data modeling

Rayleigh scattering

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