Imaging of living cells in real time

Vladimir P. Tychinsky; Georgy E. Koufal; Elena V. Perevedentseva; Tatiana V. Vyshenskaia; Serguei L. Nikandrov

doi:10.1117/12.260807

6 December 1996 Imaging of living cells in real time

Vladimir P. Tychinsky, Georgy E. Koufal, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, Serguei L. Nikandrov

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260807
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Parameters of intrinsic cell motility is one of the cell activity characteristics which can be measured in real-time. For evaluation of certain organelles velocity we propose to use high sensitivity of computer-aided phase microscope airyscan to local phase changes connected with refractive index. This method is based on periodical scanning of cell profile in direction perpendicular to organelles movement. Analysis of the obtained 2-dimensional time-coordinate matrix allows us to define organelle velocity in quasi-real time and areas of cell activity. The experiments with onion cells confirm the method applicability for cell activity investigation.

Citation Download Citation

Vladimir P. Tychinsky, Georgy E. Koufal, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, and Serguei L. Nikandrov "Imaging of living cells in real time", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260807

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