Paper
10 April 1998 Light scattering from metal sol labels on high-density DNA probe arrays
Mark O. Trulson, Ian D. Walton, Audrey D. Suseno, Hajime Matsuzaki, David Stern
Author Affiliations +
Proceedings Volume 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery; (1998) https://doi.org/10.1117/12.307333
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States
Abstract
We have been exploring the use of light scattering as a means to detect the binding of nucleic acids to high density DNA probe arrays. Initial work has concentrated on the use of 100 nanometer gold particles conjugated to monoclonal antibodies. A probe array scanner that utilizes an arc lamp source and a `photocopier grade' linear CCD detector has been developed. The optical configuration of the scanner maximizes dynamic range and minimizes optical backgrounds. Initial development of light scattering detection for the p53 cancer gene application shows that functional performance may be obtained that is essentially equivalent to existing fluorescence detection methodology.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mark O. Trulson, Ian D. Walton, Audrey D. Suseno, Hajime Matsuzaki, and David Stern "Light scattering from metal sol labels on high-density DNA probe arrays", Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); https://doi.org/10.1117/12.307333
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Cited by 2 scholarly publications.
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KEYWORDS
Particles

Light scattering

Luminescence

Scattering

Reflection

Gold

Scanners

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