Diagnostics of photosynthesizing organisms by linear and nonlinear fluorimetry

Viktor V. Fadeev; Elena M. Filippova; D. V. Maslov; D. N. Matorin; Pavel S. Venediktov

doi:10.1117/12.364186

16 September 1999 Diagnostics of photosynthesizing organisms by linear and nonlinear fluorimetry

Viktor V. Fadeev, Elena M. Filippova, D. V. Maslov, D. N. Matorin, Pavel S. Venediktov

Proceedings Volume 3821, Environmental Sensing and Applications; (1999) https://doi.org/10.1117/12.364186
Event: Industrial Lasers and Inspection (EUROPTO Series), 1999, Munich, Germany

Abstract

In the paper, the possibilities of classic and laser fluorimetry in photosynthesizing organisms (PSO) diagnostics are analyzed. Some special features of fluorescence of PSO excited by laser pulses are discussed. The results of research presented in the paper refer to the water PSO -- phytoplankton. However, some of them are valid for vegetation. Methods of linear and non-linear fluorimetry make it possible to determine the following parameters. (a) Fluorescence parameter (Phi) _o equals N_fl^o/N_RS, where N_fl^o is the photons number of photosynthesizing organism fluorescence without saturation effect, N_RS is the number of Raman scattering photons from water molecules. (b) Parameter of photosynthetic activity (eta) equals F_v/F_m, where F_v and F_m are the intensities of variable and maximum fluorescence response. This parameter can be determined by the pump-and-probe technique. (c) Excitation cross-section of fluorophor (sigma) . This parameter depends on energy transfer form auxiliary pigments and reflects the group and species composition of algae. The cross-section (sigma) and other photophysical parameters of PSO can be determined by non-linear saturation spectroscopy. In principle, these parameters can be used for the diagnostics of PSO, i.e. for the determination of chlorophyll-a concentration, of photosynthesis efficiency and of phytoplankton species composition. In the paper the examples of spatial distributions of parameters (Phi) _o, N_fl^o and (eta) obtained by ship lidar and by submersible biophysical probe are represented. The results obtained in some sea expeditions using lidars and double-flash submersible fluorimeter for phytoplankton diagnostics, are briefly analyzed.

Citation Download Citation

Viktor V. Fadeev, Elena M. Filippova, D. V. Maslov, D. N. Matorin, and Pavel S. Venediktov "Diagnostics of photosynthesizing organisms by linear and nonlinear fluorimetry", Proc. SPIE 3821, Environmental Sensing and Applications, (16 September 1999); https://doi.org/10.1117/12.364186

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