Flow cytometry systems for drug discovery and development

John T. Ransom; Bruce S. Edwards; Frederick W. Kuckuck III; Alex Okun; David K. Mattox; Eric R. Prossnitz; Larry A. Sklar

doi:10.1117/12.384201

27 April 2000 Flow cytometry systems for drug discovery and development

John T. Ransom, Bruce S. Edwards, Frederick W. Kuckuck III, Alex Okun, David K. Mattox, Eric R. Prossnitz, Larry A. Sklar

Author Affiliations +

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384201
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

HT-PS is a fluidics-based pharmacology platform that uses viable cells and test compounds to rapidly identify active compounds and immediately determine their potency and specificity. Axiom employs this proprietary flow-through fluidics system coupled to a flow cytometer (FCM) as a detection system. Integration of FCM was enabled through a Plug-Flow Coupler (PFC) device that allows mixtures of cells and test compounds to be delivered to the FCM as discrete plugs of samples under positive air pressure. An FCM detector provides the advantages of multi parametric measurements and multiplexed, single cell analyses. Assays that combine two or more compatible, fluorescent bioresponse indicators simultaneously, such as measurements of intracellular pH and Ca²⁺, are possible. Alternatively, measurements of one or more bioresponses can be performed on several distinct cell populations individually stained with uniquely addressable fluorescent chromophores. These formats enable multiple experiments on a single sample and provide high content information thereby greatly increasing decision-making power regarding the activity, potency and selectivity of a test compound. Development of significant data with several hundred cells enables reduction in all requisite sample volumes. The PFC enables FCM sample analysis rates of at least 10 samples/minute. The data will illustrate HT-PS/PFC/FCM utility in the drug discovery arena.

Citation Download Citation

John T. Ransom, Bruce S. Edwards, Frederick W. Kuckuck III, Alex Okun, David K. Mattox, Eric R. Prossnitz, and Larry A. Sklar "Flow cytometry systems for drug discovery and development", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384201

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