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A new technique of focused ion beam (FIB) microscopy/nanomachining is proposed. Ultrahigh resolution 3D tomography can be performed on whole cell, without preparation. Very fast imaging times allow quasi real time microscopy. FIB is a source of ions which can be precisely oriented and focused on the sample. The resolution can be as low as 15 nm. Higher currents produce 'cutting' and 'attaching' operations of very high precision where at the same time the resulting secondary ions and electrons again provide follow up sample imaging.
Marziale Milani,Monica Ballerini, andFranco Squadrini
"Focused ion beam: moving toward nanobiotechnology", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383351
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Marziale Milani, Monica Ballerini, Franco Squadrini, "Focused ion beam: moving toward nanobiotechnology," Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383351