In-vitro correlation between reduced scattering coefficient and hemoglobin concentration of human blood determined by near-infrared spectroscopy

Lelia Adelina Paunescu; Antonios Michalos; Jee Hyun Choi; Ursula Wolf; Martin Wolf; Enrico Gratton

doi:10.1117/12.434505

29 June 2001 In-vitro correlation between reduced scattering coefficient and hemoglobin concentration of human blood determined by near-infrared spectroscopy

Lelia Adelina Paunescu, Antonios Michalos, Jee Hyun Choi, Ursula Wolf, Martin Wolf, Enrico Gratton

Author Affiliations +

Proceedings Volume 4250, Optical Tomography and Spectroscopy of Tissue IV; (2001) https://doi.org/10.1117/12.434505
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We study the correlation between (mu) _s' and THC obtained in vitro, in a highly scattering medium containing human blood. We used a frequency domain near infrared spectrometer (modulation frequency: 110 MHz, wavelengths: 758 and 830 nm) to measure in real time (acquisition time: 0.64 s) (mu) _s' and THC. We used Liposyn suspension and red blood cells in saline buffer solution. After a couple of minutes of baseline acquisition, several consecutive increments of 3-5 ml blood were added to the solution yielding THC equals 15-100 (mu) M and (mu) _a equals 0.03-0.3 1/cm. At the last amount of blood added, increments of glucose in the range of 0.5-20 g/L were added. For each step of blood and glucose added, data were acquired for a couple of minutes. This was repeated 6 times. Average of data was calculated for both (mu) _s' and THC for each of the red blood cells and glucose increments added. We found a high correlation between (mu) _s' and THC (0.018 X THC + 4.51, R² equals 0.98 at 758 nm and 0.012 X THC + 4.86, R² equals 0.97 at 830 nm). We studied the effect of glucose on (mu) _s' and we found a high correlation between the glucose added to the suspension and the decrease in (mu) _s' for the case of high glucose concentrations. The slope of this correlation is -0.011 at both wavelengths and the correlation factors were R² X 0.96 at 830 nm and R² equals 0.91 at 758 nm (case shown). The effect of glucose was less significant at 830 nm than at 758 nm in general. This work is a proof of principle for detection of (mu) _s' changes with glucose. This approach also establishes limits for glucose detection in physiological conditions.

Citation Download Citation

Lelia Adelina Paunescu, Antonios Michalos, Jee Hyun Choi, Ursula Wolf, Martin Wolf, and Enrico Gratton "In-vitro correlation between reduced scattering coefficient and hemoglobin concentration of human blood determined by near-infrared spectroscopy", Proc. SPIE 4250, Optical Tomography and Spectroscopy of Tissue IV, (29 June 2001); https://doi.org/10.1117/12.434505

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available