Dynamic studies on living cells with an atomic force fluorescence microscope

Jens Struckmeier; Erk Klopp; Matthias Born; Damaris Rink; Jochen Tenbosch; Stefan Ecke; Hans-Juergen Butt; Martin R. Hofmann; David B. Jones

doi:10.1117/12.426764

10 May 2001 Dynamic studies on living cells with an atomic force fluorescence microscope

Jens Struckmeier, Erk Klopp, Matthias Born, Damaris Rink, Jochen Tenbosch, Stefan Ecke, Hans-Juergen Butt, Martin R. Hofmann, David B. Jones

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Proceedings Volume 4260, Optical Diagnostics of Living Cells IV; (2001) https://doi.org/10.1117/12.426764
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Analysis of the reaction of bone structure to mechanical stimulation is a key issue in understanding the origins of osteoporosis and mechanical adaptation of living bone to external forces. This is thought to be regulated on a cellular level. We have investigated quantitative mechanical stimulation of single bone cells and their immediate intracellular calcium responses using a combination of an atomic force microscope (AFM) and a fluorescence microscope, developed in our laboratory. The force stimulation system can apply quantified forces in the pico- and nano-newton regime on exactly defined positions of a cell. We present here the first measurements using this system on the mechanically induced free calcium response of primary osteoblasts. The threshold forces for stimulation and the temporal behavior of the free calcium response are analyzed and the signal transmission to adjacent cells is investigated. An immediate increase of the intracellular calcium concentration is observed after dynamic stimulation with forces between 110nN and 550nN. Forces exceeding 700nN destroy the cell and the free calcium concentration of the neighboring cells react as a consequence.

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Jens Struckmeier, Erk Klopp, Matthias Born, Damaris Rink, Jochen Tenbosch, Stefan Ecke, Hans-Juergen Butt, Martin R. Hofmann, and David B. Jones "Dynamic studies on living cells with an atomic force fluorescence microscope", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); https://doi.org/10.1117/12.426764

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KEYWORDS

Calcium

Luminescence

Bone

Optical spheres

Microscopes

Atomic force microscopy

Calibration

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