Fluorescence method for monitoring of glucose in interstitial fluids

Maxim V. Loshchenov; Alexandr S. Parfenov; Gennady L. Kisselev; Alexander A. Stratonnikov; Katerina Ershova; Rudolf W. Steiner

doi:10.1117/12.429344

13 June 2001 Fluorescence method for monitoring of glucose in interstitial fluids

Maxim V. Loshchenov, Alexandr S. Parfenov, Gennady L. Kisselev, Alexander A. Stratonnikov, Katerina Ershova, Rudolf W. Steiner

Author Affiliations +

Proceedings Volume 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring; (2001) https://doi.org/10.1117/12.429344
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Development of minimal invasive diagnostics methods is of great importance in today's healthcare. The present work deals with the measurements of glucose concentration in small drops, its volume being in the range of 1 (mu) L and its glucose concentration being in the range of 1-10 mM. The aim of the work was to develop robust quantitative method of glucose measurement in small volumes of interstitial fluid obtained after laser shot or by other low invasive procedures. The commercially available Amlex Red glucose assay kit was used in the present work. The glucose is detected by enzyme-coupling reactions resulting in formation of fluorescence dye resorufin. The diagnostic paper was used as a reaction medium. The fluorescence of dye in diagnostic paper was measured with the use of fiber optics spectrometer. It was shown that dye fluorescence correlates fairly well with glucose concentration. The method may be modified to involve cholesterol concentration measurement as well.

Citation Download Citation

Maxim V. Loshchenov, Alexandr S. Parfenov, Gennady L. Kisselev, Alexander A. Stratonnikov, Katerina Ershova, and Rudolf W. Steiner "Fluorescence method for monitoring of glucose in interstitial fluids", Proc. SPIE 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring, (13 June 2001); https://doi.org/10.1117/12.429344

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available