Joint optimization of lighting and sensing for the CCD-type DNA chip scanner

Jong Dae Kim; Jin-Hee Kim; Jongwon Kim

doi:10.1117/12.424588

16 April 2001 Joint optimization of lighting and sensing for the CCD-type DNA chip scanner

Jong Dae Kim, Jin-Hee Kim, Jongwon Kim

Proceedings Volume 4264, Genomics and Proteomics Technologies; (2001) https://doi.org/10.1117/12.424588
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

While the DNA chip is going to be prevalent for the medical diagnostics and drug development, its analysis tool such as a chip scanner need to be optimized. To obtain the guideline for the optimized CCD-based scanner, this paper addresses the minimum requirements for the selected CCD-based detector under the chosen light condition. The scope of our experiments was focused on the scanning performance rather than the design of the chip scanner itself. Through the gray level analysis of the target spot and background, we could check the possibility of the CCD-based detector and the lighting lamp to adopt them as a low price scanner. A quality measure was investigated with two levels of lighting power and the various integration times. For the CCD-based detector and the Xenon lighting system, we evaluated the image performance with the developed quality measure. We define a background and a sample area mask manually to investigate the statistics of the images captured by the scanner. The developed system is composed with a medium price CCD camera and a zenon lamp lighting system. The developed system has reasonable performance for the scanned image. The risk ofphotobleaching was not shown in our experiments.

Citation Download Citation

Jong Dae Kim, Jin-Hee Kim, and Jongwon Kim "Joint optimization of lighting and sensing for the CCD-type DNA chip scanner", Proc. SPIE 4264, Genomics and Proteomics Technologies, (16 April 2001); https://doi.org/10.1117/12.424588

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available