Novel photobleaching model in living cells

Tongsheng Chen; Shaoqun Zeng; Qingming Luo; Wei Zhou; Zhihong Zhang

doi:10.1117/12.438419

22 August 2001 Novel photobleaching model in living cells

Tongsheng Chen, Shaoqun Zeng, Qingming Luo, Wei Zhou, Zhihong Zhang

Proceedings Volume 4427, Imaging of Tissue Structure and Function; (2001) https://doi.org/10.1117/12.438419
Event: Imaging of Tissue Structure and Function, 2001, none, Russian Federation

Abstract

A novel photobleaching model was proposed, in which a D-P photobleaching mechanism was developed. By combining with the conventional D-D and D-O photobleaching mechanisms, the dependence of photobleaching rate on the excitation power is well illustrated. The validity of this model was demonstrated with GFP photobleaching experiments in cases of one-photon excitation (1PE) and two-photon excitation (2PE) respectively. Previously inexplicable experimental results published in literature were also illustrated with this new model.

Citation Download Citation

Tongsheng Chen, Shaoqun Zeng, Qingming Luo, Wei Zhou, and Zhihong Zhang "Novel photobleaching model in living cells", Proc. SPIE 4427, Imaging of Tissue Structure and Function, (22 August 2001); https://doi.org/10.1117/12.438419

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