Three-dimensional imaging method for biological samples under liquid

Kee S. Moon; Kah W. Ng

doi:10.1117/12.444105

4 October 2001 Three-dimensional imaging method for biological samples under liquid

Kee S. Moon, Kah W. Ng

Proceedings Volume 4564, Optomechatronic Systems II; (2001) https://doi.org/10.1117/12.444105
Event: Intelligent Systems and Advanced Manufacturing, 2001, Boston, MA, United States

Abstract

This paper presents a new non-contact method for measuring 3-D features of biological materials. The method can provide 3-D images of samples under liquid such as water with a microscopic resolution. One of the advantages of measuring under liquid includes protecting air sensitive surfaces of biological materials. The method is based on optical microscopy with machine vision and precision actuation. The geometric information of 3-D samples can be obtained by analyzing the focus errors of the image pixels. Experimental results have been presented and discussed to demonstrate the technique.

Citation Download Citation

Kee S. Moon and Kah W. Ng "Three-dimensional imaging method for biological samples under liquid", Proc. SPIE 4564, Optomechatronic Systems II, (4 October 2001); https://doi.org/10.1117/12.444105

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