System for holographic interferometry based on bacteriorhodopsin films

Norbert A. Hampp; Thorsten Juchem

doi:10.1117/12.446653

25 October 2001 System for holographic interferometry based on bacteriorhodopsin films

Norbert A. Hampp, Thorsten Juchem

Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446653
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore

Abstract

Films made from genetically modified bacteriorhodopsins (BR) are attractive media for holographic recording. A holographic camera system is presented which uses BR-films as photowritable-photoerasable optical recording media. This camera system is useful in applications like non-destructive testing, vibration analysis and size measurement. To our knowledge this is the first technical system were a biological material, the BR-films, are employed as a performance determining component. The camera head is dust proof sealed and internally damped for applications in a technical environment. All control and display devices are mounted in a separate 19' rack which comes with the system. The system can be easily switched between various holographic measurement techniques under software control, among them double-exposure, time-averaging, real-time and phase-shifting interferometry. The system is able to resolve deformations with a resolution down to 5 nm ((lambda) /100) and operates at 12 frames/second. A variable read-out magnification allows to take advantage of the very high resolution of the BR-films which is beyond 5000 lines/mm. Polarization recording in BR-films is employed to obtain a high signal-to-noise ratio even at low exposures of 100 (mu) J/cm². The recording mechanism at low light levels is discussed in detail.

Citation Download Citation

Norbert A. Hampp and Thorsten Juchem "System for holographic interferometry based on bacteriorhodopsin films", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); https://doi.org/10.1117/12.446653

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