Effective axial superresolution in single-photon 4Pi microscopy by Toraldo rings

Manuel Martinez-Corral; M. T. Caballero; Amparo Pons Marti

doi:10.1117/12.526975

19 November 2003 Effective axial superresolution in single-photon 4Pi microscopy by Toraldo rings

Manuel Martinez-Corral, M. T. Caballero, Amparo Pons Marti

Author Affiliations +

Proceedings Volume 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life; (2003) https://doi.org/10.1117/12.526975
Event: 19th Congress of the International Commission for Optics: Optics for the Quality of Life, 2002, Florence, Italy

Abstract

We demonstrate theoretically the feasibility of single-photon 4Pi-confocal microscopy. By inserting a pair of properly designed multi-ring phase-only pupil filters in the illumination path of a 4Pi microscope the height of the sidelobes of the point spread function is importantly reduced, so that there is not ambiguity in the 3D image. Then, an axial resolution up to four times higher than that of single-photon confocal microscope can be effectively achieved.

Citation Download Citation

Manuel Martinez-Corral, M. T. Caballero, and Amparo Pons Marti "Effective axial superresolution in single-photon 4Pi microscopy by Toraldo rings", Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); https://doi.org/10.1117/12.526975

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