Injection of glycerol into porcine skin for optical skin clearing with needle-free injection gun and determination of agent distribution using OCT and fluorescence microscopy

Oliver Stumpp; Ashley J. Welch

doi:10.1117/12.476147

12 September 2003 Injection of glycerol into porcine skin for optical skin clearing with needle-free injection gun and determination of agent distribution using OCT and fluorescence microscopy

Oliver Stumpp, Ashley J. Welch

Author Affiliations +

Proceedings Volume 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII; (2003) https://doi.org/10.1117/12.476147
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

The aim of this study is to investigate the feasability of using a needle-free injection gun to inject highly viscous glycerol into porcine skin for the purpose of optical skin clearing. In this study a needle-free injection device DERMO-JET by Robbins Instruments was used to inject glycerol in 100%, 50% and 25% concentration into freshly excised porcine skin. The injection sites were imaged using optical coherence tomography (OCT) amplitude data to determine injection depth. Similar experiments were conducted involving the same device for injection of 100%, 50% and 25% glycerol-water mixtures with trace amounts of rhodamine. Injected tissue was rapidly frozen and sectioned with a keratome to reveal the mixture dispersion with a fluorescence microscope.

Citation Download Citation

Oliver Stumpp and Ashley J. Welch "Injection of glycerol into porcine skin for optical skin clearing with needle-free injection gun and determination of agent distribution using OCT and fluorescence microscopy", Proc. SPIE 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, (12 September 2003); https://doi.org/10.1117/12.476147

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available