Studies of cochlear outer hair cell membrane mechanics using optical tweezers

David R Murdock; Sergey A. Ermilov; William E. Brownell; Bahman Anvari

doi:10.1117/12.476469

12 September 2003 Studies of cochlear outer hair cell membrane mechanics using optical tweezers

David R Murdock, Sergey A. Ermilov, William E. Brownell, Bahman Anvari

Proceedings Volume 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII; (2003) https://doi.org/10.1117/12.476469
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

An optical tweezers system was used to study the mechanical characteristics of outer hair cell (OHC) and human embryonic kidney (HEK) cell plasma membranes. The effect of the cationic amphipath chlorpromazine (CPZ) on the equilibrium tethering force, (F_eq) force relaxation time constant,(τ) and effective membrane viscosity (η_eff) was measured. The F_eq for the OHC lateral wall plasma membrane was ~60 pN and was unchanged by addition of CPZ. A significantly greater τ value was observed in CPZ-treated OHCs (30.5 ± 12.6 s) than in control OHCs (19.0 ± 13.2 s). The F_eq and τ values for control HEK cells were >60% lower than the respective OHC values but increased by ~3 times following CPZ addition. Effective viscosity ranged between 1.49-1.81 pN•s/μm for CPZ-treated OHCs. This represents a decrease from reported control OHC membrane viscosities.

Citation Download Citation

David R Murdock, Sergey A. Ermilov, William E. Brownell, and Bahman Anvari "Studies of cochlear outer hair cell membrane mechanics using optical tweezers", Proc. SPIE 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, (12 September 2003); https://doi.org/10.1117/12.476469

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