C60 nanoparticle quenching used as a biological label

Jun Feng; Sally Mack; Guomin Shan; Shirley Gee; Bruce D. Hammock; Ian M. Kennedy

doi:10.1117/12.478397

12 September 2003 C₆₀ nanoparticle quenching used as a biological label

Jun Feng, Sally Mack, Guomin Shan, Shirley Gee, Bruce D. Hammock, Ian M. Kennedy

Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications; (2003) https://doi.org/10.1117/12.478397
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Carbon 60 has been used in a functionalized form in a bioassay for a common herbicide, atrazine. It was found that the C₆₀ is a very effective quencher of fluorescence from a number of common dyes. C₆₀ was conjugated to atrazine for use in an immunoassay in which fluorescence from rhodamine was measured. Quenching of the rhodamine emission provided a detection scheme in this assay that yielded a very good limit of detection. The C₆₀ quenching scheme can be used with a wide variety of fluorescent dyes, permitting the potential use of a range of small, cheap excitation sources.

Citation Download Citation

Jun Feng, Sally Mack, Guomin Shan, Shirley Gee, Bruce D. Hammock, and Ian M. Kennedy "C₆₀ nanoparticle quenching used as a biological label", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); https://doi.org/10.1117/12.478397

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