The application of novel multifunctional microarray slides for immobilization biomolecules

Youxiang Wang; Yaping Zong; Jennifer Shi; Shannon Zhang

doi:10.1117/12.519793

22 June 2004 The application of novel multifunctional microarray slides for immobilization biomolecules

Youxiang Wang, Yaping Zong, Jennifer Shi, Shannon Zhang

Proceedings Volume 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II; (2004) https://doi.org/10.1117/12.519793
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Microarray has revolutionized the study of molecular biology, especially the application in clinical diagnostics. When used in clinical diagnostics, microarray has to meet a high degree of reproducibility, reliability and quality in order to become a standard tool. Repeatability and reproducibility are essential for providing the best data and process control. The real challenge for microarray is, however, how to produce consistent and reliable data. The variance of microarray data is contributed by the quality of sample source, the quality of glass substrates, hybridization, and probe labeling and spotting. The quality of coated glass substrates is one of the main factors. This paper is focusing on discussing how to optimize coating conditions to improve the slide quality, consequently improve the data quality such as sensitivity and data reliability.

Citation Download Citation

Youxiang Wang, Yaping Zong, Jennifer Shi, and Shannon Zhang "The application of novel multifunctional microarray slides for immobilization biomolecules", Proc. SPIE 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, (22 June 2004); https://doi.org/10.1117/12.519793

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