Microscopic birefringence imaging by the local-sampling phase shifting technique

Mizue Ebisawa; Yukitoshi Otani; Norihiro Umeda

doi:10.1117/12.547768

10 September 2004 Microscopic birefringence imaging by the local-sampling phase shifting technique

Mizue Ebisawa, Yukitoshi Otani, Norihiro Umeda

Proceedings Volume 5462, Biophotonics Micro- and Nano-Imaging; (2004) https://doi.org/10.1117/12.547768
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

A microscopic birefringence imaging of bio-sample is proposed. This system consists of a super luminescent diode (SLD), polarizers, a quarter-wave plate and a phase retarder. The instrument is provided to map and visualize an optical anisotropy in bio-sample. A local-sampling phase shifting technique is employed for analytic algorithm with high resolution of retardance. A Bereck compensator is used a sample for checking its accuracy. Birefringence distributions of gelatin orientation such as retardance and azimuthal direction are shown in case of applied voltage and changing temperature as its demonstration. It is possible to observe molecular orientation of bio-sample.

Citation Download Citation

Mizue Ebisawa, Yukitoshi Otani, and Norihiro Umeda "Microscopic birefringence imaging by the local-sampling phase shifting technique", Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); https://doi.org/10.1117/12.547768

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