Attenuation of excitation and emission path of epifluorescence microscope

Grzegorz Jerzy Kopec

doi:10.1117/12.577168

26 August 2004 Attenuation of excitation and emission path of epifluorescence microscope

Grzegorz Jerzy Kopec

Proceedings Volume 5566, Optical Security and Safety; (2004) https://doi.org/10.1117/12.577168
Event: Optical Security and Safety, 2003, Warsaw, Poland

Abstract

Spectral properties of excitation and emission path of epifluorescence microscope at object and image planes has been determined and analyzed for two of filter sets. Attenuation of excitation path at object plane and attenuation of emission path at image plane were proved to be useful for this purpose. Calculations were carried out for epifluorescence filters made for FITC (fluorescein) type fluorophores (494ex/518em).

Citation Download Citation

Grzegorz Jerzy Kopec "Attenuation of excitation and emission path of epifluorescence microscope", Proc. SPIE 5566, Optical Security and Safety, (26 August 2004); https://doi.org/10.1117/12.577168

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