Raman molecular chemical imaging: 3D Raman using deconvolution

John S. Maier M.D.; Patrick J. Treado

doi:10.1117/12.571512

7 December 2004 Raman molecular chemical imaging: 3D Raman using deconvolution

Proceedings Volume 5588, Smart Medical and Biomedical Sensor Technology II; (2004) https://doi.org/10.1117/12.571512
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

Chemical imaging is a powerful technique combining molecular spectroscopy and digital imaging for rapid, non-invasive and reagentless analysis of materials, including biological cells and tissues. Raman chemical imaging is suited to the characterization of molecular composition and structure of biomateials at submicron spatial resolution (< 250 nm). As a result, Raman imaging has potential as a routine tool for the assessment of cells and subcellular components. In this presentation, we discuss Raman chemical imaging and spectroscopy of single human cells obtained from a culture line. Rapid three dimensional Raman imaging is shown using deconvolution to improve image quality.

Citation Download Citation

John S. Maier M.D. and Patrick J. Treado "Raman molecular chemical imaging: 3D Raman using deconvolution", Proc. SPIE 5588, Smart Medical and Biomedical Sensor Technology II, (7 December 2004); https://doi.org/10.1117/12.571512

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