Imaging of protein kinase C activation by FRET during proliferation induced by low-energy laser irradiation in living cells

Xuejuan Gao; Tongsheng Chen; Da Xing; Fang Wang

doi:10.1117/12.572780

18 January 2005 Imaging of protein kinase C activation by FRET during proliferation induced by low-energy laser irradiation in living cells

Xuejuan Gao, Tongsheng Chen, Da Xing, Fang Wang

Author Affiliations +

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.572780
Event: Photonics Asia, 2004, Beijing, China

Abstract

Protein kinase Cs (PKCs) play an important role in cellular proliferation, and low-energy laser irradiation (LELI) can enhance cellular proliferation. The present work contributes to the understanding of the mechanisms of action by studying effects of LELI at the dose of 0.8 J/cm² on PKCs activities in the single lung adenocarcinoma cell (ASTC-a-1) and in real time by fluorescence resonance energy transfer (FRET) technique. C-kinase activity reporter (CKAR), consisting of a cyan fluorescent protein (CFP), the FHA2 phosphothreonine-binding domain, a PKC substrate sequence, and a yellow fluorescent protein (YFP), was utilized. The living cell imaging showed a decrease in FRET in the cytosol and nucleus after the cells were treated with LELI. These results suggest that PKCs could be activated by LELI throughout the cell, and the proliferation of ASTC-a-1 cells could be modulated by the activated PKCs.

Citation Download Citation

Xuejuan Gao, Tongsheng Chen, Da Xing, and Fang Wang "Imaging of protein kinase C activation by FRET during proliferation induced by low-energy laser irradiation in living cells", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.572780

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