Pulsed photodynamic inactivation of gram-negative bacteria: electron microscopic study

Masafumi Nagasawa; Shunichi Sato; Satoko Kawauchi; Nariyoshi Shinomiya; Daizo Saito; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.589993

8 April 2005 Pulsed photodynamic inactivation of gram-negative bacteria: electron microscopic study

Masafumi Nagasawa, Shunichi Sato, Satoko Kawauchi, Nariyoshi Shinomiya, Daizo Saito, Hiroshi Ashida, Minoru Obara

Proceedings Volume 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV; (2005) https://doi.org/10.1117/12.589993
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We previously demonstrated the methylene blue-mediated photodynamic inactivation of P. aeruginosa in vitro using pulsed light excitation. However, the photobactericidal mechanism has not been revealed yet. In this study, morphological changes induced by pulsed photodynamic treatment in bacteria were investigated by electron microscopy. The micrographs of bacteria showed an alteration in the membrane structure in the early period of illumination, and increase in the total light dose caused an alteration in the cytoplasmic structure. It was shown that the membrane damage may be lethal to bacteria.

Citation Download Citation

Masafumi Nagasawa, Shunichi Sato, Satoko Kawauchi, Nariyoshi Shinomiya, Daizo Saito, Hiroshi Ashida, and Minoru Obara "Pulsed photodynamic inactivation of gram-negative bacteria: electron microscopic study", Proc. SPIE 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV, (8 April 2005); https://doi.org/10.1117/12.589993

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