Monitoring apoptosis of TK-GFP-expressing ACC-M cells induced by ACV using FRET technique

Tao Xiong; Zhihong Zhang; Juqiang Lin; Jie Yang; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.710962

27 October 2006 Monitoring apoptosis of TK-GFP-expressing ACC-M cells induced by ACV using FRET technique

Tao Xiong, Zhihong Zhang, Juqiang Lin, Jie Yang, Shaoqun Zeng, Qingming Luo

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472J (2006) https://doi.org/10.1117/12.710962
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Apoptosis is an evolutionary conserved cellular process that plays an important role during development, but it is also involved in tissue homeostasis and in many diseases. To study the characteristics of suicide gene system of the herpes simplex virus thymidine kinase (HSV-tk) gene in tumor cells and explore the apoptosis phenomena in this system and its effect on the human adenoid cystic carcinoma line ACC-M cell, we detected apoptosis of CD3- (ECFP-CRS-DsRed) and TK-GFP-expressing ACC-M (ACC-M-TK-GFP-CD3) cells induced by acyclovir (ACV) using fluorescence resonance energy transfer (FRET) technique. CD3 is a FRET-based indicator for activity of caspase-3, which is composed of an enhanced cyan fluorescent protein, a caspase-3 sensitive linker, and a red fluorescent protein from Discosoma with efficient maturation property. FRET from ECFP to DsRed could be detected in normal ACC-M-TK-GFP-CD3 cells, and the FRET efficient was remarkably decreased and then disappeared during the cells apoptosis induced by ACV. It was due to the activated caspase-3 cleaved the CD3 fusion protein. In this study, the results suggested that the AVC-induced apoptosis of ACC-M-TK-GFP-CD3 cells was through caspase-3 pathway.

Citation Download Citation

Tao Xiong, Zhihong Zhang, Juqiang Lin, Jie Yang, Shaoqun Zeng, and Qingming Luo "Monitoring apoptosis of TK-GFP-expressing ACC-M cells induced by ACV using FRET technique", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472J (27 October 2006); https://doi.org/10.1117/12.710962

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Fluorescence resonance energy transfer

Cell death

Tumors

Fluorescent proteins

Green fluorescent protein

Luminescence

Microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years