Dynamic imaging of interaction between protein 14-3-3 and Bid in living cells

Tongsheng Chen; Da Xing; Jinjun Wang

doi:10.1117/12.644517

24 February 2006 Dynamic imaging of interaction between protein 14-3-3 and Bid in living cells

Tongsheng Chen, Da Xing, Jinjun Wang

Proceedings Volume 6087, Biophotonics and Immune Responses; 60870L (2006) https://doi.org/10.1117/12.644517
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The 14-3-3 proteins are known to sequester certain pro-apoptotic members of this family. BH3- interacting domain death agonist (Bid) may contribute to tumor necrosis factor α(TNF-α)-induced neuronal death, although regulation by 14-3-3 has not been reported. In this study we examined whether 14-3-3 proteins interact with Bid/tBid during TNF-α-induced cell death. The TNF-αtriggered Bid cleavage and tBid translocated to mitochondria. Human lung adenocarcinoma cells were co-transfected with both CFP-Bid and 14-3-3-YFP plasmids, and the dynamical interaction between the Bid/tBid and 14-3-3 were performed on laser confocal fluorescence microscope in single living cell during TNF-α-induced cell apoptosis. The Bid distribute equally only in the cytoplasm of healthy cells, and the 14-3-3 protein distribute not only in the cytoplasm but also in the nucleus of healthy cells. Our data showed that the tBid aggregate, but the 14-3-3 protein does not aggregate as the tBid, and the 14-3-3 protein separate from the aggregated tBid, implying that the 14-3-3 proteins do not interact with the aggregated tBid after TNF-αtreatment.

Citation Download Citation

Tongsheng Chen, Da Xing, and Jinjun Wang "Dynamic imaging of interaction between protein 14-3-3 and Bid in living cells", Proc. SPIE 6087, Biophotonics and Immune Responses, 60870L (24 February 2006); https://doi.org/10.1117/12.644517

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