Rapid Raman spectroscopy of musculoskeletal tissue using a visible laser and an electron-multiplying CCD (EMCCD) detector

Kurtulus Golcuk; Gurjit S. Mandair; Andrew F. Callender; William F. Finney; Nadder Sahar; David H. Kohn; Michael D. Morris

doi:10.1117/12.646209

27 February 2006 Rapid Raman spectroscopy of musculoskeletal tissue using a visible laser and an electron-multiplying CCD (EMCCD) detector

Kurtulus Golcuk, Gurjit S. Mandair, Andrew F. Callender, William F. Finney, Nadder Sahar, David H. Kohn, Michael D. Morris

Author Affiliations +

Proceedings Volume 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry; 609314 (2006) https://doi.org/10.1117/12.646209
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Background fluorescence can often complicate the use of Raman microspectroscopy in the study of musculoskeletal tissues. Such fluorescence interferences are undesirable as the Raman spectra of matrix and mineral phases can be used to differentiate between normal and pathological or microdamaged bone. Photobleaching with the excitation laser provides a non-invasive method for reducing background fluorescence, enabling 532 nm Raman hyperspectral imaging of bone tissue. The signal acquisition time for a 400 point Raman line image is reduced to 1-4 seconds using electronmultiplying CCD (EMCCD) detector, enabling acquisition of Raman images in less than 10 minutes. Rapid photobleaching depends upon multiple scattering effects in the tissue specimen and is applicable to some, but not all experimental situations.

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Kurtulus Golcuk, Gurjit S. Mandair, Andrew F. Callender, William F. Finney, Nadder Sahar, David H. Kohn, and Michael D. Morris "Rapid Raman spectroscopy of musculoskeletal tissue using a visible laser and an electron-multiplying CCD (EMCCD) detector", Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 609314 (27 February 2006); https://doi.org/10.1117/12.646209

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