Interpretation of interference signals in label free integrated interferometric biosensors

Hanna Heikkinen; Meng Wang; Matti Okkonen; Jukka Hast; Risto Myllylä

doi:10.1117/12.655465

27 February 2006 Interpretation of interference signals in label free integrated interferometric biosensors

Hanna Heikkinen, Meng Wang, Matti Okkonen, Jukka Hast, Risto Myllylä

Proceedings Volume 6094, Optical Diagnostics and Sensing VI; 60940P (2006) https://doi.org/10.1117/12.655465
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In the future fast, simple and reliable biosensors will be needed to detect various analytes from different biosamples. This is due to fact that the needs of traditional health care are changing. In the future homecare of patients and peoples' responsibility for their own health will increase. Also, different wellness applications need new parameters to be analysed, reducing costs of traditional health care, which are increasing rapidly. One fascinating and promising sensor type for these applications is an integrated optical interferometric immunosensor, which is manufactured using organic materials. The use of organic materials opens up enormous possibilities to develop different biochemical functions. In label free biosensors the measurement is based on detecting changes in refractive index, which typically are in the range of 10^-6-10^-8 [1]. In this research, theoretically generated interferograms are used to compare various signal processing methods. The goal is to develop an efficient method to analyse the interferogram. Different time domain signal processing methods are studied to determine the measuring resolution and efficiency of these methods. A low cost CCD -element is used in detecting the interferogram dynamics. It was found that in most of the signal processing methods the measuring resolution was mainly limited by pixel size. With calculation of Pearson's correlation coefficient, subpixel resolution was achieved which means that nanometer range optical path differences can be measured. This results in the refractive index resolution of the order of 10^-7.

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Hanna Heikkinen, Meng Wang, Matti Okkonen, Jukka Hast, and Risto Myllylä "Interpretation of interference signals in label free integrated interferometric biosensors", Proc. SPIE 6094, Optical Diagnostics and Sensing VI, 60940P (27 February 2006); https://doi.org/10.1117/12.655465

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KEYWORDS

Signal to noise ratio

Interferometry

Biosensors

Fringe analysis

Signal processing

Image resolution

CCD cameras

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