Immune cells tracing using quantum dots

Akiyoshi Hoshino; Kouki Fujioka; Yuki I. Kawamura; Noriko Toyama-Sorimachi; Masato Yasuhara M.D.; Taeko Dohi M.D.; Kenji Yamamoto M.D.

doi:10.1117/12.645420

27 March 2006 Immune cells tracing using quantum dots

Akiyoshi Hoshino, Kouki Fujioka, Yuki I. Kawamura, Noriko Toyama-Sorimachi, Masato Yasuhara M.D., Taeko Dohi M.D., Kenji Yamamoto M.D.

Author Affiliations +

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 609613 (2006) https://doi.org/10.1117/12.645420
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Fluorescent nanoparticles, such as nanocrystal quantum dots (QDs), have potential to be applied to molecular biology and bioimaging, since some nanocrystals emit higher and longer lasting fluorescence than conventional organic probes do. Here we report an example of labeling immune cells by QDs. We collected splenic CD4⁺ T-lymphocyte and peritoneal macrophages from mice. Then cells were labeled with QDs. QDs are incorporated into the T-lymphocyte and macrophages immediately after addition and located in the cytoplasm via endocytosis pathway. The fluorescence of QDs held in the endosomes was easily detected for more than a week. In addition, T-lymphocytes labeled with QDs were stable and cell proliferation or cytokine production including IL-2 and IFN-γ was not affected. When QD-labeled T-lymphocytes were adoptively transferred intravenously to mice, they remained in the peripheral blood and spleen up to a week. Using QD-labeled peritoneal macrophages, we studied cell traffic during inflammation on viscera in peritoneum cavity. QD-labeled macrophages were transplanted into the peritoneum of the mouse, and colitis was induced by intracolonic injection of a hapten, trinitrobenzensulfonic acid. With the aid of stong signals of QDs, we found that macrophage accumuled on the inflammation site of the colon. These results suggested that fluorescent probes of QDs might be useful as bioimaging tools for tracing target cells in vivo.

Citation Download Citation

Akiyoshi Hoshino, Kouki Fujioka, Yuki I. Kawamura, Noriko Toyama-Sorimachi, Masato Yasuhara M.D., Taeko Dohi M.D., and Kenji Yamamoto M.D. "Immune cells tracing using quantum dots", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 609613 (27 March 2006); https://doi.org/10.1117/12.645420

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available