Non-destructive micro-patterning of protein crystals by focused femtosecond laser

Y. Hosokawa; T. Kaji; Y. Hiraki; H. Mori; H. Masuhara

doi:10.1117/12.654706

28 February 2006 Non-destructive micro-patterning of protein crystals by focused femtosecond laser

Y. Hosokawa, T. Kaji, Y. Hiraki, H. Mori, H. Masuhara

Proceedings Volume 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI; 610805 (2006) https://doi.org/10.1117/12.654706
Event: Lasers and Applications in Science and Engineering, 2006, San Jose, California, United States

Abstract

Micro-patterning of proteins has been attracted much attention as a potential technique to realize bio-microdevice. In this work, as a new method to realize non-destructive micro-patterning of proteins, laser transfer printing for a um-sized protein crystal was developed by utilizing focused femtosecond laser. The micro-patterning was performed to transfer the protein crystal which was adhered on a source substrate to a target substrate which was underlaid on the source substrate. An 800-nm femtosecond laser was focused in a water between the source and target substrates on an inverted microscope with a 100x objective lens. When the laser focal point was scanned at the position with distance of a few um far from the source substrate, the protein crystals were detached by a shockwave and cavitation bubble generation at the circumstance of the focal point and transferred to the target substrate forming a line pattern. The line width of the protein crystal was a few tens um with the scanning speed of 90 μm/sec. Furthermore, multi-patterning of several kinds of protein crystals was realized by this method. The pattering resolution is comparable or better than that by another multi-material transfer printing, such as ink jet printing, micro-printing, and laser direct writing.

Citation Download Citation

Y. Hosokawa, T. Kaji, Y. Hiraki, H. Mori, and H. Masuhara "Non-destructive micro-patterning of protein crystals by focused femtosecond laser", Proc. SPIE 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI, 610805 (28 February 2006); https://doi.org/10.1117/12.654706

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available