A combined remote Raman and fluorescence spectrometer system for detecting inorganic and biological materials

Shiv K. Sharma; Anupam K. Misra; Paul G. Lucey

doi:10.1117/12.693868

12 December 2006 A combined remote Raman and fluorescence spectrometer system for detecting inorganic and biological materials

Shiv K. Sharma, Anupam K. Misra, Paul G. Lucey

Author Affiliations +

Proceedings Volume 6409, Lidar Remote Sensing for Environmental Monitoring VII; 64090K (2006) https://doi.org/10.1117/12.693868
Event: SPIE Asia-Pacific Remote Sensing, 2006, Goa, India

Abstract

We have developed a combined remote telescopic Raman and laser-induced native fluorescence (LINF) spectrograph with 532 nm pulsed laser excitation and a gated CCD detector. With this system, we have measured time-resolved Raman and LINF spectral measurements at 9 m with 10-ns time resolution. A comparison of Raman spectra of calcite crystal and that of chicken eggshell show that the CaCO₃ in the chicken eggshell is arranged in a calcite structure. The strong LINF band in the spectrum of the calcite crystal has lifetime longer than 1 μs, whereas the lifetime of LINF bands of the eggshell are in 10's of nano-sec (ns). The time-resolved Raman spectra of tomato and poinsettia (Euphorbia pulcherrimum) green leaves show resonance Raman features of carotenes. The time-resolved remote LINF spectrum of ruby crystals, and LINF spectra of tomato and poinsettia green leaves yield information that the LINF lifetime of ruby lines is much longer (in milliseconds (ms)) as compared with the fluorescence lifetime of the tomato and the poinsettia leaves (in 10s of ns). These results show that it will be possible to discriminate between inorganic and biogenic materials on the basis of LINF lifetimes even with 8 nano-sec laser pulses and gated detection.

Citation Download Citation

Shiv K. Sharma, Anupam K. Misra, and Paul G. Lucey "A combined remote Raman and fluorescence spectrometer system for detecting inorganic and biological materials", Proc. SPIE 6409, Lidar Remote Sensing for Environmental Monitoring VII, 64090K (12 December 2006); https://doi.org/10.1117/12.693868

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available