In vivo multiphoton imaging of bile duct ligation

Yuan Liu; Feng-Chieh Li; Hsiao-Chin Chen; Po-shou Chang; Shu-Mei Yang; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/12.764264

15 February 2008 In vivo multiphoton imaging of bile duct ligation

Yuan Liu, Feng-Chieh Li, Hsiao-Chin Chen, Po-shou Chang, Shu-Mei Yang, Hsuan-Shu Lee, Chen-Yuan Dong

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68600G (2008) https://doi.org/10.1117/12.764264
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Bile is the exocrine secretion of liver and synthesized by hepatocytes. It is drained into duodenum for the function of digestion or drained into gallbladder for of storage. Bile duct obstruction is a blockage in the tubes that carry bile to the gallbladder and small intestine. However, Bile duct ligation results in the changes of bile acids in serum, liver, urine, and feces1, 2. In this work, we demonstrate a novel technique to image this pathological condition by using a newly developed in vivo imaging system, which includes multiphoton microscopy and intravital hepatic imaging chamber. The images we acquired demonstrate the uptake, processing of 6-CFDA in hepatocytes and excretion of CF in the bile canaliculi. In addition to imaging, we can also measure kinetics of the green fluorescence intensity.

Citation Download Citation

Yuan Liu, Feng-Chieh Li, Hsiao-Chin Chen, Po-shou Chang, Shu-Mei Yang, Hsuan-Shu Lee, and Chen-Yuan Dong "In vivo multiphoton imaging of bile duct ligation", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68600G (15 February 2008); https://doi.org/10.1117/12.764264

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