Label-free in vivo imaging of Drosophila melanogaster by multiphoton microscopy

Chiao-Ying Lin; Vladimir Hovhannisyan; June-Tai Wu; Sung-Jan Lin; Chii-Wann Lin; Jyh-Horng Chen; Chen-Yuan Dong

doi:10.1117/12.763202

15 February 2008 Label-free in vivo imaging of Drosophila melanogaster by multiphoton microscopy

Chiao-Ying Lin, Vladimir Hovhannisyan, June-Tai Wu, Sung-Jan Lin, Chii-Wann Lin, Jyh-Horng Chen, Chen-Yuan Dong

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 686019 (2008) https://doi.org/10.1117/12.763202
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

The fruit fly Drosophila melanogaster is one of the most valuable organisms in genetic and developmental biology studies. Drosophila is a small organism with a short life cycle, and is inexpensive and easy to maintain. The entire genome of Drosophila has recently been sequenced (cite the reference). These advantages make fruit fly an attractive model organism for biomedical researches. Unlike humans, Drosophila can be subjected to genetic manipulation with relative ease. Originally, Drosophila was mostly used in classical genetics studies. In the model era of molecular biology, the fruit fly has become a model organ for developmental biology researches. In the past, numerous molecularly modified mutants with well defined genetic defects affecting different aspects of the developmental processes have been identified and studied. However, traditionally, the developmental defects of the mutant flies are mostly examined in isolated fixed tissues which preclude the observation of the dynamic interaction of the different cell types and the extracellular matrix. Therefore, the ability to image different organelles of the fruit fly without extrinsic labeling is invaluable for Drosophila biology. In this work, we successfully acquire in vivo images of both developing muscles and axons of motor neurons in the three larval stages by using the minimially invasive imaging modality of multiphoton (SHG) microscopy. We found that while SHG imaging is useful in revealing the muscular architecture of the developing larva, it is the autofluorescence signal that allows label-free imaging of various organelles to be achieved. Our results demonstrate that multiphoton imaging is a powerful technique for investigation the development of Drosophila.

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Chiao-Ying Lin, Vladimir Hovhannisyan, June-Tai Wu, Sung-Jan Lin, Chii-Wann Lin, Jyh-Horng Chen, and Chen-Yuan Dong "Label-free in vivo imaging of Drosophila melanogaster by multiphoton microscopy", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 686019 (15 February 2008); https://doi.org/10.1117/12.763202

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KEYWORDS

Multiphoton microscopy

Microscopy

Second-harmonic generation

Biology

Genetics

Organisms

In vivo imaging

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