Antibody fragment recognition layers for surface plasmon resonance biosensing: a parametric study

André Magalhães; Sandro Bordeira; Ana Cristina Almeida; Vanessa Fontes; Maria João Leite Costa; Luís P. Fonseca; João Garcia da Fonseca

doi:10.1117/12.808820

23 February 2009 Antibody fragment recognition layers for surface plasmon resonance biosensing: a parametric study

André Magalhães, Sandro Bordeira, Ana Cristina Almeida, Vanessa Fontes, Maria João Leite Costa, Luís P. Fonseca, João Garcia da Fonseca

Author Affiliations +

Proceedings Volume 7169, Advanced Biomedical and Clinical Diagnostic Systems VII; 716903 (2009) https://doi.org/10.1117/12.808820
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A comparative study is reported regarding the use of two different surface plasmon resonance (SPR) biosensors, a homemade SPR grating biosensor and a reference prism coupled biosensor, to perform quantification of C-reactive protein (CRP) in human blood serum. Surface functionalization was conducted using anti-CRP fragments immobilized directly on gold. Adsorption time optimization for the antibody fragments monolayer, non-specific binding (NSB) resistance evaluation and CRP detection were conducted, with better results achieved by the grating biosensor on all topics, namely less functionalization time, higher resistance to NSB and wider CRP dynamic concentration range. A study regarding comparison between continuous flow and surface coating immobilization is also reported in this work. We have shown that surface coating immobilization achieves similar NSB resistance and CRP detection results, allowing a 75% assay cost reduction by lower solution volume requirement. Results suggest that the coating immobilization technique is the best suited to be used in further studies in order to obtain a viable immunosensor for CRP and other biomarkers detection in complex biological fluids.

Citation Download Citation

André Magalhães, Sandro Bordeira, Ana Cristina Almeida, Vanessa Fontes, Maria João Leite Costa, Luís P. Fonseca, and João Garcia da Fonseca "Antibody fragment recognition layers for surface plasmon resonance biosensing: a parametric study", Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716903 (23 February 2009); https://doi.org/10.1117/12.808820

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available