Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

R.-Y. He; K.-C. Cho; N.-S. Chang; Y.-D. Su; S.-J. Chen

doi:10.1117/12.809134

13 February 2009 Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

R.-Y. He, K.-C. Cho, N.-S. Chang, Y.-D. Su, S.-J. Chen

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71831L (2009) https://doi.org/10.1117/12.809134
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Citation Download Citation

R.-Y. He, K.-C. Cho, N.-S. Chang, Y.-D. Su, and S.-J. Chen "Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831L (13 February 2009); https://doi.org/10.1117/12.809134

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