In-situ analysis of fruit anthocyanins by means of total internal reflectance, continuous wave and time-resolved spectroscopy

Manuela Zude; Lorenzo Spinelli; Carsten Dosche; Alessandro Torricelli

doi:10.1117/12.823157

10 September 2009 In-situ analysis of fruit anthocyanins by means of total internal reflectance, continuous wave and time-resolved spectroscopy

Manuela Zude, Lorenzo Spinelli, Carsten Dosche, Alessandro Torricelli

Author Affiliations +

Proceedings Volume 7432, Optical Inspection and Metrology for Non-Optics Industries; 74320H (2009) https://doi.org/10.1117/12.823157
Event: SPIE Optical Engineering + Applications, 2009, San Diego, California, United States

Abstract

In sweet cherry (Prunus avium), the red pigmentation is correlated with the fruit maturity stage and can be measured by non-invasive spectroscopy. In the present study, the influence of varying fruit scattering coefficients on the fruit remittance spectrum (cw) were corrected with the effective pathlength and refractive index in the fruit tissue obtained with distribution of time-of-flight (DTOF) readings and total internal reflection fluorescence (TIRF) analysis, respectively. The approach was validated on fruits providing variation in the scattering coefficient outside the calibration sample set. In the validation, the measuring uncertainty when non-invasively analyzing fruits with cw method in comparison with combined application of cw, DTOF, and TIRF measurements showed an increase in r² up to 22.7 % with, however, high errors in all approaches.

Citation Download Citation

Manuela Zude, Lorenzo Spinelli, Carsten Dosche, and Alessandro Torricelli "In-situ analysis of fruit anthocyanins by means of total internal reflectance, continuous wave and time-resolved spectroscopy", Proc. SPIE 7432, Optical Inspection and Metrology for Non-Optics Industries, 74320H (10 September 2009); https://doi.org/10.1117/12.823157

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