New strategies to measure intracellular sodium concentrations

Sascha Dietrich; Sarmiza E. Stanca; Charles G. Cranfield; Birgit Hoffmann; Klaus Benndorf; Christoph Biskup

doi:10.1117/12.849742

2 March 2010 New strategies to measure intracellular sodium concentrations

Sascha Dietrich, Sarmiza E. Stanca, Charles G. Cranfield, Birgit Hoffmann, Klaus Benndorf, Christoph Biskup

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756914 (2010) https://doi.org/10.1117/12.849742
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Fluorescent ion indicators are widely used to measure ion concentrations in living cells. However, despite considerable efforts in synthesizing new compounds, no ratiometric sodium indicator is available that can be excited at visible wavelengths. Ratiometric indicators have an advantage in that measured fluorescence intensities can be corrected for fluctuations of the indicator concentration and the illumination intensity, which is not possible when non-ratiometric indicators are used. One way to circumvent this problem is to measure fluorescence lifetimes, which are independent of these factors. Another way to overcome the disadvantages of a non-ratiometric indicator dye is to embed it, together with a reference dye, into nanoparticles. By relating the indicator fluorescence to the fluorescence of the reference dye, inhomogeneities in the nanosensor concentration or the illumination intensity can be cancelled out reliably. In this study we compare the benefits and drawbacks of these approaches.

Citation Download Citation

Sascha Dietrich, Sarmiza E. Stanca, Charles G. Cranfield, Birgit Hoffmann, Klaus Benndorf, and Christoph Biskup "New strategies to measure intracellular sodium concentrations", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756914 (2 March 2010); https://doi.org/10.1117/12.849742

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