Annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for contrast improvement

Jian Lin; Fake Lu; Haifeng Wang; Wei Zheng; C. J. R. Sheppard; Zhiwei Huang

doi:10.1117/12.842942

26 February 2010 Annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for contrast improvement

Jian Lin, Fake Lu, Haifeng Wang, Wei Zheng, C. J. R. Sheppard, Zhiwei Huang

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756939 (2010) https://doi.org/10.1117/12.842942
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We report on a unique annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy to effectively remove the solvent background for high contrast vibrational imaging. Our finite-difference time-domain (FDTD) calculations show that the far-field RP-CARS radiation from the scatterer with size comparable to the excitation wavelength is stronger than that from the solvent at large cone angles (around 45° to 150°). The annular detection provides about one order higher contrast for both forward and backward detected RP-CARS microscopy.

Citation Download Citation

Jian Lin, Fake Lu, Haifeng Wang, Wei Zheng, C. J. R. Sheppard, and Zhiwei Huang "Annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for contrast improvement", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756939 (26 February 2010); https://doi.org/10.1117/12.842942

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