Optical coherence tomography speckle decorrelation for detecting cell death

Golnaz Farhat; Adrian Mariampillai; Victor X. D. Yang; Gregory J. Czarnota; Michael C. Kolios

doi:10.1117/12.874682

11 February 2011 Optical coherence tomography speckle decorrelation for detecting cell death

Golnaz Farhat, Adrian Mariampillai, Victor X. D. Yang, Gregory J. Czarnota, Michael C. Kolios

Author Affiliations +

Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 790710 (2011) https://doi.org/10.1117/12.874682
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We present a dynamic light scattering technique applied to optical coherence tomography (OCT) for detecting changes in intracellular motion caused by cellular reorganization during apoptosis. We have validated our method by measuring Brownian motion in microsphere suspensions and comparing the measured values to those derived based on particle diffusion calculated using the Einstein-Stokes equation. Autocorrelations of OCT signal intensities acquired from acute myeloid leukemia cells as a function of treatment time demonstrated a significant drop in the decorrelation time after 24 hours of cisplatin treatment. This corresponded with nuclear fragmentation and irregular cell shape observed in histological sections. A similar analysis conducted with multicellular tumor spheroids indicated a shorter decorrelation time in the spheroid core relative to its edges. The spheroid core corresponded to a region exhibiting signs of cell death in histological sections and increased backscatter intensity in OCT images.

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Golnaz Farhat, Adrian Mariampillai, Victor X. D. Yang, Gregory J. Czarnota, and Michael C. Kolios "Optical coherence tomography speckle decorrelation for detecting cell death", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790710 (11 February 2011); https://doi.org/10.1117/12.874682

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