Spectral and lifetime domain measurements of rat brain tumours

D. Abi Haidar; B. Leh; K. Allaoua; A. Genoux; R. Siebert; M. Steffenhagen; D. Peyrot; N. Sandeau; C. Vever-Bizet; G. Bourg-Heckly; I. Chebbi; M. Collado-Hilly

doi:10.1117/12.907411

9 February 2012 Spectral and lifetime domain measurements of rat brain tumours

D. Abi Haidar, B. Leh, K. Allaoua, A. Genoux, R. Siebert, M. Steffenhagen, D. Peyrot, N. Sandeau, C. Vever-Bizet, G. Bourg-Heckly, I. Chebbi, M. Collado-Hilly

Author Affiliations +

Proceedings Volume 8207, Photonic Therapeutics and Diagnostics VIII; 82074P (2012) https://doi.org/10.1117/12.907411
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

During glioblastoma surgery, delineation of the brain tumour margins remains difficult especially since infiltrated and normal tissues have the same visual appearance. This problematic constitutes our research interest. We developed a fibre-optical fluorescence probe for spectroscopic and time domain measurements. First measurements of endogenous tissue fluorescence were performed on fresh and fixed rat tumour brain slices. Spectral characteristics, fluorescence redox ratios and fluorescence lifetime measurements were analysed. Fluorescence information collected from both, lifetime and spectroscopic experiments, appeared promising for tumour tissue discrimination. Two photon measurements were performed on the same fixed tissue. Different wavelengths are used to acquire two-photon excitation-fluorescence of tumorous and healthy sites.

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D. Abi Haidar, B. Leh, K. Allaoua, A. Genoux, R. Siebert, M. Steffenhagen, D. Peyrot, N. Sandeau, C. Vever-Bizet, G. Bourg-Heckly, I. Chebbi, and M. Collado-Hilly "Spectral and lifetime domain measurements of rat brain tumours", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82074P (9 February 2012); https://doi.org/10.1117/12.907411

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