Three-dimensional ultrahigh-resolution optical coherence tomography imaging of lung tissues

S. Ishida; N. Nishizawa; M. Kitatsuji; H. Ohshima; M. Matsushima; T. Kawabe

doi:10.1117/12.2003514

8 March 2013 Three-dimensional ultrahigh-resolution optical coherence tomography imaging of lung tissues

S. Ishida, N. Nishizawa, M. Kitatsuji, H. Ohshima, M. Matsushima, T. Kawabe

Proceedings Volume 8565, Photonic Therapeutics and Diagnostics IX; 856531 (2013) https://doi.org/10.1117/12.2003514
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We have been investigating ultrahigh resolution optical coherence tomography (UHR-OCT) imaging of lung tissues using fiber based super continuum (SC) sources. The high power, low-noise, Gaussian shaped SC generated with ultrashort pulses and optical fibers at several wavelength regions were used as the broadband light sources for UHROCT. Since the lung consists of tiny alveoli which are separeted by thin wall, the UHR-OCT is supposed to be effective for lung imaging. The normal and diseased lung tissues were observed without invasive procedures to the lung itself. The clear images of alveoli were observed with index matching effect by saline. In this work, we investigated the three-dimensional UHR-OCT imaging of lung structure. The lungs of rats inflated with 10% formalin at 5 cmH₂O, 15 cmH₂O, and 20 cmH₂O pressure were prepared as the sample for investigation of size and shape of the lung structure. These samples were fixed with 10% formalin. The interalveolar septa, thin walls separating the alveoli, were clearly observed. The difference of size and shape of alveoli and thier three-dimensional network was clearly observed from the UHR-OCT images. The clear images of alveoli were observed with index matching effect of 10% formalin. We investigated the wavelength dependence of 3D UHR-OCT image of lung structure at 800 nm, 1060 nm, and 1700 nm wavelength regions. The 3D UHR-OCT images of structure of rat lung were clearly observed in all wavelength regions and wavelength dependence of imaging was discussed.

Citation Download Citation

S. Ishida, N. Nishizawa, M. Kitatsuji, H. Ohshima, M. Matsushima, and T. Kawabe "Three-dimensional ultrahigh-resolution optical coherence tomography imaging of lung tissues", Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 856531 (8 March 2013); https://doi.org/10.1117/12.2003514

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available