A new expanded point information content design approach for 3D live-cell microscopy at video rates

Ramzi N. Zahreddine; Robert H. Cormack; Carol J. Cogswell

doi:10.1117/12.2006493

22 February 2013 A new expanded point information content design approach for 3D live-cell microscopy at video rates

Ramzi N. Zahreddine, Robert H. Cormack, Carol J. Cogswell

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 858902 (2013) https://doi.org/10.1117/12.2006493
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Through a combination of optical design and algorithm development, a new expanded point information content (EPIC) microscope has been developed that is capable of extending the depth of field while simultaneously super locating the depth position of complex biological objects to within an accuracy of 75 nm. The data is then combined to form 3D animations of live-cell biological specimens. This is accomplished without the need to acquire multi-focal image stacks and is thus well suited for high-speed imaging.

Citation Download Citation

Ramzi N. Zahreddine, Robert H. Cormack, and Carol J. Cogswell "A new expanded point information content design approach for 3D live-cell microscopy at video rates", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858902 (22 February 2013); https://doi.org/10.1117/12.2006493

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