Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopy

H. Salehi; E. Middendorp; A-G. Végh; S-K. Ramakrishnan; C. Gergely; F. J. G. Cuisinier

doi:10.1117/12.1000149

19 February 2013 Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopy

H. Salehi, E. Middendorp, A-G. Végh, S-K. Ramakrishnan, C. Gergely, F. J. G. Cuisinier

Author Affiliations +

Proceedings Volume 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X; 85940F (2013) https://doi.org/10.1117/12.1000149
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Confocal Raman Microscopy, a non-invasive, label free imaging technique is used to study apoptosis in living MCF-7 cells. The images are based on Raman spectra of cells components. K-mean clustering was used to determine mitochondria position in cells and cytochrome c distribution inside the cells was based on correlation analysis. Cell apoptosis is defined as cytochrome c diffusion in cytoplasm. Co-localization of cytochrome c is found within mitochondria after three hours of incubation with 10 μM paclitaxel. Our results demonstrate that the presence of paclitaxel at this concentration in the culture media for 3 hours does not induce apoptosis of MCF7 cells via a caspase independent pathway.

Citation Download Citation

H. Salehi, E. Middendorp, A-G. Végh, S-K. Ramakrishnan, C. Gergely, and F. J. G. Cuisinier "Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopy", Proc. SPIE 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940F (19 February 2013); https://doi.org/10.1117/12.1000149

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available